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. 2022 Dec 16;10(12):2496. doi: 10.3390/microorganisms10122496

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Growth and production of short-chain fatty acids (SCFAs) during cultivation of Bifidobacterium adolescentis (BA) and Roseburia hominis (RH) on (galacto)-β-mannan-oligosaccharide (MOS/GMOS). (A) Cell concentration by qPCR-analysis for BA and RH in mono- and cocultures. (B) Relative strain abundance (%) in the coculture. (C) The BA monocultivation (in the medium for colon bacteria, MCB). (D) The RH monocultivation (in the modified medium for colon bacteria, mMCB). (E) The cocultivation of RH and BA in MCB. RecA (for BA) and rho (for RH) genes were used as markers in the qPCR-analysis to determine the cell concentration, expressed as log (copy number/mL) in the mono- and cocultures. The sum of calculated gene-copy-number values was used to determine the relative population (%) of RH and BA in the cocultures. The optical density (OD) was determined at 600 nm. The amount of SCFAs was determined by HPLC.

Growth and production of short-chain fatty acids (SCFAs) during cultivation of Bifidobacterium adolescentis (BA) and Roseburia hominis (RH) on (galacto)-β-mannan-oligosaccharide (MOS/GMOS). (A) Cell concentration by qPCR-analysis for BA and RH in mono- and cocultures. (B) Relative strain abundance (%) in the coculture. (C) The BA monocultivation (in the medium for colon bacteria, MCB). (D) The RH monocultivation (in the modified medium for colon bacteria, mMCB). (E) The cocultivation of RH and BA in MCB. RecA (for BA) and rho (for RH) genes were used as markers in the qPCR-analysis to determine the cell concentration, expressed as log (copy number/mL) in the mono- and cocultures. The sum of calculated gene-copy-number values was used to determine the relative population (%) of RH and BA in the cocultures. The optical density (OD) was determined at 600 nm. The amount of SCFAs was determined by HPLC.