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. 2022 Dec 7;37(16):1111–1129. doi: 10.1089/ars.2021.0215

FIG. 4.

FIG. 4.

Intracerebral hemorrhage induces AHR overexpression and transcriptional activity in the ipsilateral hemisphere in mice. (A) Temporal expression of AHR with representative Western blot bands and quantitative analysis of AHR at 6, 24, 48, 72 h and 7 days in the ICH group. n = 6/per group. (B) Double immunofluorescence staining for AHR (green) with neurons (NeuN, red), microglia (Iba-1, red), and astrocytes (GFAP, red) around the hematoma at 48 h in different groups after ICH. The brain slice on the right of top panel indicates the location of staining quantification (small black box). Scale bar = 50 μm, n = 4 for each group. (C, D) Representative Western blot bands and quantitative analysis of nuclear/cytoplasm AHR ratio at 24, 48, and 72 h in the ICH group. n = 4/per group. (E) Representative Western blot bands and quantitative analysis of CYP1A1 at 6, 24, 48, 72 h and 7 days in the ICH group. n = 6/per group. The error bars represent mean ± SD. *p < 0.05. **p < 0.01. Kruskal–Wallis test (Wilcoxon signed-rank test), Tukey's post hoc test. GFAP, glial fibrillary acidic protein; IBA-1, ionized calcium binding adaptor molecule-1.