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. 2022 Dec 7;37(16):1111–1129. doi: 10.1089/ars.2021.0215

FIG. 8.

FIG. 8.

Deleterious AHR-dependent effect of kynurenine in ICH. (A) Temporal generation of endogenous kynurenine after ICH. n = 6/per group, Kruskal–Wallis test (Wilcoxon signed-rank test), Tukey's post hoc test. The error bars represent mean ± SD. *p < 0.05. **p < 0.01. (B, C) Neurobehavioral testing using modified Garcia test and forelimb placing test in different groups. n = 6/per group, Kruskal–Wallis test (Wilcoxon signed-rank test), Tukey's post hoc test. The error bars represent mean ± SD. *p < 0.05. **p < 0.01. (D–F) Representative microphotographs and quantitative analysis for FJC, TUNEL staining in the ipsilateral mice brain in different groups at 48 h after ICH. Brain slice on the right top panel indicates the location of staining quantification (small black box). Upper panel: Immunofluorescence staining for FJC-positive cells (green) with nuclear (DAPI, blue), scale bar = 100 μm. Lower panel: Immunofluorescence staining for neuron (NeuN, red) with TUNEL-positive cells (green), scale bar = 100 μm. Kruskal–Wallis test (Wilcoxon signed-rank test), Tukey's test. n = 6 for each group. The error bars represent mean ± SD. *p < 0.05. **p < 0.01. (G, H) Representative Western blot bands and quantitative analysis of total AHR and nuclear/cytoplasm AHR ratio at 48 h in different ICH group. n = 4–6/per group. Kruskal–Wallis test (Wilcoxon signed-rank test), Tukey's test. The error bars represent mean ± SD. *p < 0.05. **p < 0.01. (I, J) Representative microphotographs and quantitative analysis for 8-OHdG staining in the ipsilateral mice brain in different groups at 48 h after ICH. Eight-OHdG-positive cells (green) with nuclear (DAPI, blue), scale bar = 50 μm. One-way ANOVA, Tukey's test. n = 6 for each group. The error bars represent mean ± SD. *p < 0.05. **p < 0.01.