Table 1.

Conventional and nested PCR protocols used in the present study.

Target Gene	Primers	Fragment Size (bp)	References
18S rRNA	1st reaction: BTF1 (5′-GGCTCATTACAACAGTTATAG-3′) and BTR21 (5′-CCCAAAGACTTTGATTTCTCTC-3′); 2nd reation: BTF2 (5′-CCGTGCTAATTGTAGGGCTAATAC-3′) and BTR2 (5′-GGACTACGACGGTATCTGATCG-3′).	~800	[13]
18S rRNA	Nbab_1F (5′- AAGCCATGCATGTCTAAGTATAAGCTTTT-3′) and 18Sapir (5′-GGATCACTCGATCGGTAGGAG-3′)	~1500	[23,24]
18S rRNA	1st reaction: Piro0F (5′-GCCAGTAGTCATATGCTTGTGTTA-3′) and Piro6R (5′-CTCCTTCCTYTAAGTGATAAGGTTCAC-3′); 2nd reaction: Piro1F (5′-CCATGCATGTCTWAGTAYAARCTTTTA-3′) and Piro5.5R (5′-CCTYTAAGTGATAAGGTTCACAAAACTT-3′)	~1500	[22]
cox-1	1st reaction: Bab_for1 (5′-ATWGGATTYTATATGAGTAT-3′) and Bab_Rev1 (5′-ATAATCWGGWATYCTCCTTGG-3′), Bab_for2; 2nd reation: (5′-TCTCTWCATGGWTTAATTATGATAT-3′) and Bab_Rev2 (5′-TAGCTCCAATTGAHARWACAAAGTG-3′)	~924	[14,15]
cox-3	COX3F (5′-ACTGTCAGCTAAAACGTATC-3′) and COX3R (5′-ACAGGATTAGATACCCTGG-3′)	~600	[16,17]
hsp70	hsp70F1 (5′-CATGAAGCACTGGCCHTTCAA-3′) and hsp70R2 (5′-GBAGGTTGTTGTCCTTVGTCAT-3)	~740	[18]
cytb	cytbF (5′-TTAGTGAAGGAACTTGACAGGT-3′) and cytbR (5′-CGGTTAATCTTTCCTATTCCTTACG-3′)	~1000	[16,17]
β-tubulin	Tubu-63F (5′-CAAATWGGYGCMAARTTYTGGGA-3′) and Tubu-3F (5′-TCGTCCATACCTTCWCCSGTRTACCAGTG-3′)	~1200	[19]
ITS-1	1st reaction: ITS15C (5′-CGATCGAGTGATCCGGTGAATTA-3′) and ITS13B (5′-GCTGCGTCCTTCATCGTTGTG-3′); 2nd reaction: (5′-AAGGAAGGAGAAGTCGTAACAAGG-3′) and ITS15C (5′-TTGTGTGAGCCAAGACATCCA-3′)	~450	[20]
ema-1	1st reaction: EMAE-F (5′-CCGCCCTTCACCTCGTTCTCAA-3′) and EMAE-R (5′-TCTCGGCGGCATCCTTGACCTC-3′); 2nd reaction: EMAI-F (5′-CCGTCTCCGTTGACTTGGCCG-3′) and EMAIR (5′-GGACGCGCTTGCCTGGAGCCT-3′)	~396	[21]