Table 2.

Set of primers used for the sequencing of near-full length 18S rRNA gene.

Primers	Primers	Annealing Temperature	Primers Reference
Pair 1	Piro1F (5′-CCA TGC ATG TCT WAG TAY AAR CTT TTA-3′) and Piro5.5R (5′-CCT YTA AGT GAT AAG GTT CAC AAA ACT T-3′)	59 °C	[26]
Pair 2	BabF2 (5′-CCG TGC TAA TTG TAG GGC TAA TAC A-3′) and BabR2 (5′-GCT TGA AAC ACT CTA RTT TTC TCA A-3′)	59 °C	[18]
Single 1	Bab2F2 (5′-CTT TGA GAA ATT AGA GTG TTT-3′)	59 °C	Present study