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. 2022 Dec 1;10(12):2066. doi: 10.3390/vaccines10122066

Figure 2.

Figure 2

Amplification of the NP (left) and M2e (right) genes in a gradient PCR. Lanes M, 1 kb DNA markers; 1, 55 °C; 2, 58.9 °C; 3, 62.5 °C; 4, 67.2 °C and 5, 71 °C. The amplification yield of the NP gene (~1.5 kb) decreased with the increasing annealing temperatures whereas the M2e gene (132 bp) was only amplified at 71 °C.