Table 2.

Primers used in PCR site-directed mutagenesis.

Primer	Sequence
Forward 1F(M2e forward primer)	5′-ATA AAG GAT CCG AGT CTT CTA ACC GAG GTC GAA ACG CCT ACC AGA AAC GAA-3′
Reverse 1R	5′-GCC TTG AGA CGC ACC ACC ACT ACC ACC ATT TGC GGC AAC AGC AAT AGG-3
Forward 2F	5′-GCT GTT GCC GCA AAT GGT GGT AGT GGT GGT GCG TCT CAA GGC ACC-3′
Reverse 2R	5′-ACT CTT ATG TGC TGG ATT CTC ATT TGG TCT AAT-3′
Forward 3F	5′-GAG AAT CCA GCA CAT AAG AGT CAA TTA GTG TGG-3′
Reverse 3R	5′-GGA GTC CAT TGC CTC CAT GTT CTC-3′
Forward 4F	5′-GAG AAC ATG GAG GCA ATG GAC TCC-3′
Reverse 4R	5′-TAC CGA GAA AGT GGG CTG AAC GCT-3′
Forward 5F	5′-AGC GTT CAG CCC ACT TTC TCG GTA-3′
Reverse 5R	5′-ACT TTC CAT CAT TCT TAT GAT TTC-3′
Forward 6F	5′-GAA ATC ATA AGA ATG ATG GAA AGT-3′
Reverse 6R(NP reverse primer)	5′-TCA AGC TTC ATT GTC ATA CTC CTC TGC-3′