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Development of a novel middle-down proteomic assay. (A) Overview of middle-down MS assay. (B) Overview of isolation, fragmentation, and PRM of phosphorylated precursors for relative quantification of isomers, an example is given for a diphosphorylated tail. (C) Sequence of human mGluR2 (accession #Q14416) cleavage product and potential phosphorylation sites (red) used for PRM studies. Residue numbering in (C) is based on the endogenous sequence reported in accession #Q14416-01.
