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Chi-square (χ2) test for QTL segregation in backcross generations.
| Generation | QTL | QTL +ve plants | QTL −ve plants | Total no. plants | Observed ratio | Expected ratio | Total χ2 value at p = 0.05 | ||
|---|---|---|---|---|---|---|---|---|---|
| BC1F1 | qANTH | 377 | 383 | 760 | 1:1 | 1:1 | 0.0473 | ||
| qGY(s) | 374 | 386 | 760 | 1:1 | 1:1 | 0.1924 | |||
| qANTH+ qGY(s) | 187 | 187 | 190 | 196 | 760 | 1:1:1:1 | 1:1:1:1 | 0.2834 | |
| BC2F1 | qANTH | 169 | 187 | 356 | 1:1 | 1:1 | 0.9111 | ||
| qGY(s) | 167 | 189 | 356 | 1:1 | 1:1 | 1.3594 | |||
| qANTH+ qGY(s) | 85 | 85 | 88 | 97 | 356 | 1:1:1:1 | 1:1:1:1 | 1.1114 | |
“s” denotes stress condition.
