Figure 1.

(A,B) Cryo-TEM of niclosamide ASD nanoparticles after 24 h of dialysis in niclosamide-supersaturated water (5 mL of the sample over 1 L of water) to remove excess inorganic and bile salts. (C) Even after the 24 h dialysis, these particles were covered by a bilayer membrane corona of ~4.5 nm. The samples had a niclosamide concentration of 256.60 ± 15.60 µg/mL (measured before vitrification).