Table 3.
Sequences of the oligonucleotides used in the qRT-PCR experiments.
| Gene | Oligonucleotide Sequence | Annealing Temperature (°C) |
|---|---|---|
| gyrA | fw 1 5′-ATTGTTGCTCGGGCTCTTCCAG-3′ rv 2 5′-ATGCGGCTTGTCAGGAGTAACC-3′ |
62 |
| bip | fw 5′-TGCGGTCTATTGACCTCCTC-3′ rv 5′- CGGGGTACCTTGATTATTTA-3′ |
60 |
| vicK | fw 5′-CACTTTACGCATTCGTTTTGCC-3′ rv 5′-CGTTCTTCTTTTTCCTGTTCGGTC-3′ |
52 |
| comC | fw 5′-CCAAAATGGTATTATGGCTGTCG-3′ rv 5′-TGAGTCTCTATCAAAGTAACGCAG-3′ |
60 |
| comD | fw 5′-GCGCTATTCCTGCAAACTCG-3’ rv 5′-TGACTTGTTTAGGCGGGCAA-3′ |
60 |
| comE | fw 5′-TTCAATGCGGTGGGAGAACT-3′ rv 5′-GGACTGGAAGTAGCCAATCAGA-3′ |
60 |
1 fw: forward; 2 rv: reverse.