Table 4.
Minimum inhibitory concentrations.
| Peptide | S. mutans | S. mitis | S. oralis | S. sanguinis | P. aeruginosa | F. nucleatum | P. gingivalis |
|---|---|---|---|---|---|---|---|
| CSP1 | 30 | 25 | 25 | 21 | >50 | >50 | >50 |
| AcCSP1 | 20 | 24 | 21 | 27 | >50 | >50 | >50 |
| Ac4CSP1 | 26 | 28 | 23 | 25 | >50 | >50 | >50 |
| CSP1hβE | 20 | 20 | 20 | 20 | >50 | >50 | >50 |
| CSP1AA | 27 | 29 | 29 | 27 | >50 | >50 | >50 |
| CSP1Y(SO3) | 20 | 20 | 20 | 20 | >50 | >50 | >50 |
| CHX | 0.63 | 0.50 | 0.57 | 0.69 | 1.68 | 300 | 254 |
Minimum inhibitory concentrations (MIC) were determined in different bacterial strains using the broth micro-dilution method. Bacteria were incubated for 16 h with peptides (concentration range 0–50 μM) or chlorhexidine (CHX, concentration range 0.05–500 μg/mL) as a positive control under aerobic (P. aeruginosa), microaerophilic (Streptococcus spp.), or anaerobic (F. nucleatum and P. gingivalis) conditions. Three separate experiments were performed. Data are reported as μM for peptides and as μg/mL for CHX.