FIG. 7.
Thin-layer chromatography of oral streptococci. Extracts of oral streptococci representing S. gordonii, S. sanguis, S. mitis, and S. parasanguis strains (1 μg of total protein/reaction mixture) were incubated with 1.25 mM metenkephalin for 2 h at 37°C. Shown are the metenkephalin control (lane 1) and extracts of S. gordonii strains VT264 (lane 2) and VT527 (lane 3); S. sanguis strains VT529 (lane 4) and VT528 (lane 5); S. mitis strains VT266 (lane 6) and VT267 (lane 7); S. parasanguis strains VT522 (lane 8), VT525 (lane 9), VT523 (lane 10), VT524 (lane 11), and FW213 (lane 12); and the pepO mutant VT1346 (lane 13).