Table 1.

Origin of Plasmodiophora brassicae field isolates, the related field information, and the results of pathotype classification. Isolates were collected from 10 clubroot-infested fields in Moravia, the Czech Republic, from 2016 to 2020.

					Pathotype Classification 3
Isolate	Region 1	Soil pH	Frequency of OSR in a Rotation (Years)	Mean Value of DI per Region in Clubroot-Infested Fields (%) 2	ECD Code	Somé 1996	DSI on OSR cv. Mendel
1	MR	5.61	4	11.91 ± 9.23	16/10/00	P4	0
2	MR	6.80	4		16/15/29	P2	25
3	MR	6.20	4		16/15/08	P6	20
4	MR	5.82	3		16/06/25	P3	12
5	MR	5.65	4		16/06/12	P3	0
6	MR	5.73	4		16/14/24	P3	22
7	MR	6.77	4		16/10/00	P3	4
8	MR	6.74	4		16/06/12	P3	12
9	MR	6.21	3		16/04/00	P4	0
10	MR	6.76	4		16/14/13	P3	9

¹ Region in the Czech Republic: MR: Moravia ² Mean value of the disease incidence (DI) in clubroot-infested fields in the region. The incidence of disease in each clubroot-infested field was estimated by randomly choosing 100 plants (10 plants at 10 sites in the field). The presence of any swelling or gall formation on the root was assumed to be proof of P. brassicae infection. Several galls on roots were cut to check for the presence/absence of gall weevil Ceutorhynchus assimilis. ³ Classification was done according to the differential systems of the European Clubroot Differential set (ECD; [19]) and Somé et al. [20]. A cut-off point of the disease severity index (DSI) of 25% was used to classify plant reactions as resistant or susceptible [20].