TABLE 5.
Adhesion and effect of centrifugation of different Aeromonas strains onto HEp-2 cells prior to infection
| Strain | Mean no. of bacteria/ HEp-2 cell ± SDa
|
|
|---|---|---|
| Normal | Centrifuged | |
| A. hydrophila AH-3 (wild type) | 18.3 ± 2.2 | 31.6 ± 3.0 |
| FlmB insertion mutant from AH-3 (AH-1726) | 9.4 ± 1.2 (51) | 29.6 ± 3.2 (93) |
| AH-1726 complemented with pLA-Flm | 18.0 ± 2.5 | 32.5 ± 2.3 |
| A. hydrophila O:3 (wild type) | 21.6 ± 2.4 | 42.7 ± 4.3 |
| FlmB insertion mutant from O:3 (AH-1881) | 10.1 ± 1.9 (46) | 40.8 ± 5.1 (95) |
| AH-1881 complemented with pLA-Flm | 21.9 ± 3.2 | 42.1 ± 5.2 |
| A. hydrophila O:25 (wild type) | 16.0 ± 2.4 | 29.3 ± 2.8 |
| FlmB insertion mutant from O:25 (AH-1882) | 7.7 ± 1.3 (48) | 28.6 ± 2.9 (96) |
| AH-1882 complemented with pLA-Flm | 15.7 ± 2.6 | 29.9 ± 3.1 |
| A. veronii bv. sobria AH-1 (wild type) | 32.3 ± 3.9 | 71.4 ± 10.7 |
| FlmB insertion mutant from AH-1 (AH-1883) | 18.1 ± 2.2 (56) | 67.3 ± 9.9 (94) |
| AH-1883 complemented with pLA-Flm | 31.6 ± 4.4 | 70.3 ± 9.2 |
| A. veronii bv. veronii AS-28 (wild type) | 31.7 ± 4.6 | 66.8 ± 10.2 |
| FlmB insertion mutant from AS-28 (AH-1884) | 15.5 ± 3.4 (48) | 63.3 ± 9.1 (94) |
| AH-1884 complemented with pLA-Flm | 32.4 ± 3.8 | 65.9 ± 10.0 |
a
See footnote a to Table 2.