SARS-CoV-2 serological reactivity status of white-tailed deer on Staten Island, New York, between 12 December 2021 and 30 January 2022: (a) spatial distribution of sites of collection of serum samples from white-tailed deer for assessment of serological reactivity to, and neutralization of, SARS-CoV-2; red circles indicate positive detection, and blue-filled circles indicate seronegative status; (b) SARS-CoV-2 pseudovirus neutralization assay (pVNT) with pseudotyped viruses equipped with the spike proteins of the wild-type SARS-CoV-2 B.1 lineage, or that of the Alpha, Beta, Gamma, Delta or Omicron variants. Of the sVNT positive samples, 11 out of 19 showed stronger reactivity to the spike of the wild-type SARS-CoV-2 B.1 lineage than to any of the variants. Each serum was run in duplicate in two independent experiments against each pseudovirus, to determine the 50% neutralization titer (NT50). Connecting lines indicate serum from the same individual.