Figure 7.

ZmSAG39 can promote the accumulation of reactive oxygen species (ROS). (A) Diaminobenzidine (DAB) staining. Maize leaves were stained with DAB after drought treatment. (B) Nitro-blue tetrazolium chloride (NBT) staining. Maize leaves were stained with NBT after drought treatment. (C,D) Accumulation of H₂O₂ and O₂⁻ in leaves of different lines. (E–H) Analysis of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbic acid peroxidase (APX) activity. Using Student’s t-test, asterisks indicate statistically significant differences (* p < 0.05; ** p < 0.01). Data are shown as mean ± SD from three independent experiments.