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. 2022 Dec 17;23(24):16104. doi: 10.3390/ijms232416104

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Functional analysis of BnXTH genes through heterologous expression in yeast. (a) Schematic diagram of the constructed yeast vector. p426 represents the empty vector, while p426–BnXTH1, p426–BnXTH3, p426–BnXTH6, and p426–BnXTH15 represent the recombinant p426 vectors that contained the BnXTH1, BnXTH3, BnXTH6, and BnXTH15 genes, respectively. (b) Cd-tolerance assay of BnXTH genes in transgenic yeast (Δyap1). The yeast cells were treated with 0 μM and 75 μM of Cd in an SD-URA medium for 3 days at 30 °C.