Figure 3.

Bioluminescent imaging of NFκB activity shows aromatase-inhibitor-induced upregulation with and without oophorectomy. Female NFkB-RE-luc mice were treated with letrozole following an oophorectomy procedure. Controls included sham surgery and vehicle control treatment (0.3% hydroxypropylcellulose; HPC). NFκB activity was measured on the in vivo imaging system (IVIS) approximately at the 5-week timepoint. IVIS (top) and quantitation of bioluminescent signals (bottom) demonstrate induction of NFκB activity both with and without oophorectomy relative to control mice. Values are the mean ± SEM with indicated p values calculated via one-way ANOVA with Tukey’s corrections for multiple comparisons. * p ≤ 0.05; *** p ≤ 0.001. All values of p ≤ 0.05 were considered statistically significant.