TABLE 2.

Primer sequences and amplification conditions

Oligonucleotide	Sequence (5′-3′)	Product size (bp)	Annealing conditions (temp [°C], t [s])	No. of cycles
GAPDH
Sense	TGAAGGTCGGAGTCAACGGATTTGGT	983	60, 45	22, 28, 35
Antisense	CATGTGGGCCATGAGGTCCACCAC
ENA-78
Sense	CTGTGTTGAGAGAGCTGCGTTGC	216	60, 45	30, 35, 40
Antisense	GTTTTCCTTGTTTCCACCGTCC
IL-8
Sense	ATTTCTGCAGCTCTGTGTGAA	255	55, 45	30, 35, 40
Antisense	TGAATTCTCAGCCCTCTTCAA