Figure 2.

Effect of PAW-rocket salad extract on Caco2 cell viability. Caco2 cells were treated for 5 h with increasing concentrations of polyphenols (0.01–100 mg/mL) extracted from PAW-20 (white bar) or UT (gray bar) rocket salad. Cell viability was determined by CellTiter-Glo^® Luminescent Cell Viability Assay (Promega). Control represents Caco2 cells incubated with culture medium containing 0.1% DMSO. Data are presented as the mean ± SD of relative percentage of control sample (set at 100%) obtained from two independent experiments each carried out in quadruplicate. Statistical significance was calculated by pairwise multiple comparisons for one-way ANOVA followed by Tukey’s HSD post hoc test. **: significant difference versus control p < 0.001; different letters, when reported, mean significant difference between groups (PAW-20 versus UT at different concentrations), p < 0.05.