FIG. 4.

Expression of WC1 on activated and nonactivated TCR1⁺ T cells. Shown are representative flow-cytometric dot plots of TCR1 (FL1) and CD25 (IL-2R) expression (FL2) of PBMC from an M. bovis-infected animal following 24 h of culture with PBS (control) or MBSE and corresponding histograms of WC1 expression (FL3). (a) Control (PBS) CD25 versus TCR1 expression dot plot. (b and c) Histograms showing WC1 expression of R2 and R3 gated cells within the dot plot (a), respectively. (d) Dot plot of CD25 and TCR1 coexpression in response to the MBSE antigen. (e and f) Histograms of WC1 expression by cells of gate R2 (e) and gate R3 (f) within the dot plot (d).