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. 2022 Dec 14;23(24):15915. doi: 10.3390/ijms232415915

Figure 7.

Figure 7

Validation of the differentially expressed proteins identified with the iTRAQ analysis and involved in aortic inflammation, vascular remodeling, and focal adhesion. (A) Heatmap of inflammation, vascular remodeling, and focal adhesion-related protein expression in aortic tissues in each diet group. (B) qRT-PCR analysis of the protein expression in aortic tissues in each diet group (n = 4). Data is presented as mean ± SD. Samples were pooled from three independent experiments. (C) Western blot detection of Galectin-3 (gene name: Lgals3) and VCAM1 protein expression in aortic tissue. (D) Quantification of protein expression presented as density relative to GAPDH.▲▲ p < 0.01 vs. ND; ★ p < 0.05 vs. HFD; ★★ p < 0.01 vs. HFD. ND: normal diet-fed ApoE KO mice, HFD: high fat diet-fed ApoE KO mice, HFD + LI: high fat diet without iron supplementation-fed ApoE KO mice.