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Elution parameters for the AF4-UV-MALS measurements.
| Step | Focus Flow | Tip Flow | Cross Flow | Time | |
|---|---|---|---|---|---|
| mL/min | mL/min | mL/min | Type | mL/min | |
| Focus | 3.30 | 0.20 | 3.00 | - | 3.0 |
| Elution | - | 3.50 | 3.00 | Constant | 25.0 |
| - | 3.50–0.50 | 3.00–0.10 | Exponential power 0.2 | 70.0 | |
| - | 0.50 | 0.10 | Constant | 20.0 | |
| Rinse | 0.05 | 0.05 | - | - | 0.5 |
