Figure 3.

Dose–dependent inhibition of VEEV-TC83 in endothelial cells, astrocytes and pericytes following OMA treatment. (A) Schematic representing the dosing and infection strategy. Endothelial cells (B), astrocytes (C) and pericytes (D) were plated in 96-well plates and infected with VEEV-TC83 (MOI:0.1). These cells were pretreated with culture medium containing increasing concentrations of OMA for 1 h prior to infection. After infection, the drug-containing media were added back to the cells and cells were maintained at 37 °C for 18 h. Culture supernatants were obtained at 18 h and viral load was quantified by plaque assay. Infectious viral titer is indicated as plaque forming units (PFU)/mL relative to the DMSO control sample. Data represent the average of three independent samples. Statistical analysis was performed using One-way ANOVA with Dunnett’s post-test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.