Figure 6.

Dose–dependent inhibition of VEEV TrD in the endothelial cells, astrocytes and pericytes. Endothelial cells (A), astrocytes (B) and pericytes (C) were seeded in 96-well plates. The cells were pretreated with increasing concentrations of OMA for 1 h prior to infection. DMSO was maintained as the vehicle-alone control. The drug-containing media were removed and virus-containing media (TrD at MOI: 0.1) were added to the cells for 1 h to permit infection. The virus-containing medium was replaced with drug-containing media after 1 h and cells were maintained at 37 °C for 18 h. Culture supernatants were analyzed by plaque assay and infectious titer is represented as PFU/mL. Data represent the average of three independent samples. Statistical analysis was performed using One-way ANOVA with Dunnett’s post-test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.