FIGURE 1.

Schematic diagram of the experimental setup. Three‐dimensional (3D) colonoids generated from biopsies from a healthy subject were seeded as monolayers on transwell membranes and grown for 3 days until confluence. After 3 days of differentiation, colonoid monolayers were fixed for immunofluorescence staining or stimulated with fecal supernatants for 24 h. Stimulation with phosphate‐buffered saline (PBS) and lipopolysaccharide (LPS) were used as negative and positive controls, respectively. After stimulation, RNA was isolated for gene expression analysis and culture supernatants collected for detection of cytokines. 3D, 3‐dimensional; 2D, 2‐dimensional; RNA, ribonucleic acids