Figure 3.

Collagen type II immunohistochemistry visualised by DAB chromogen reaction in 6‐day‐old unstimulated chondrifying micromass cultures (A) compared to colonies that received the mechanical loading regime for 6 consecutive days (60 min daily) (B). Scale bars, 500 μm (main images on the left) or 100 μm (smaller inserts on the right). The brown deposit represents immunopositive signals for collagen type II within the chondrogenic nodules of the micromass cultures (indicated by black arrows). Representative photomicrographs are shown. DAB, diamino benzidine.