Figure 8.

Islets of Langerhans’ increase in size following ricin injection. The mice were injected ip with 30 µg/kg of ricin, and they were sacrificed at the indicated time points. The tissue was fixed and histological examination was performed. (A) Individual islets were morphologically identified, a region of interest was drawn around the circumference, and the areas were calculated. Only a single section was analyzed per mouse. Each dot represents the area of an identified islet. Mean and SEM are indicated. The Kruskal–Wallis test was performed and the red asterisks above the groups show the results of Dunn’s multiple comparison test vs. the control group: 0.002 (**) and <0.001 (***). (B) Immunohistological staining of pancreatic islets. Tissue sections were incubated with Ab to insulin, glucagon, and an irrelevant isotype control followed by alkaline phosphatase conjugated secondary Ab and the substrate aminoethyl carbazole.