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. 2022 Nov 23;14(12):820. doi: 10.3390/toxins14120820

Table 1.

PCR primer pairs used for gene expression studies.

Oligo Name Sequence Amplicon
GAPDH 5′-AATGGTGAAGGTCGGTGTGAAC-3′ 100
5′-GTCGTTGATGGCAACAATCTCC-3′
CDK2 5′-CAGAAATGATTCCCTCCAGTGC-3′ 100
5′-GAACCACGATGAACAGACCAGAG-3′
CDK4 5′-AAGCGAATCTCTGCCTTCCG-3′ 109
5′-AGGGTTTCTCCACCAAGACTGG-3′
CyclinE 5′-TTGACCCACTGGACTCTTCACAC-3′ 100
5′-ACAGCAACCTACAACACCCGAG-3′
Glucagon 5′-GAAGACAAACGCCACTCACAGG-3′ 100
5′-TGGTGTTCATCAACCACTGCAC-3′
Glucokinase 5′-AAATCCAGGCAAGGACAGGG-3′ 100
5′-AGGGGTAGCAGCAGAATAGGTCTC-3′
GLUT1 5′-ATCCCAGCAGCAAGAAGGTGAC-3′ 100
5′-GCGTTGATGACACCAGTGTTATAGC-3′
GLUT2 5′-AGCAATGTTGGCTGCAAACAG-3′ 100
5′-ACTTCGTCCAGCAATGATGAGG-3′
GLUT4 5′-TGAGAATGACTGAGGGGCAAAAC-3′ 100
5′-GGTAACAGGGAAGAGAGGGCTAAAG-3′
INSM1 5′-CAGGTGATCCTCCTTCAGGT-3′ 102
5′-CTCTTTGTGGGTCTCCGAGT-3′
Insulin1 5′-CAGCAAGCAGGTCATTGTTTCAAC-3′ 100
5′-CAAAAGCCTGGGTGGGTTTG-3′
Nkx-2.2 5′-CCCCATTCCTTTCCTTAAACCC-3′ 100
5′-CCCGCAATTTATGCCACAAAG-3′
Nkx-6.1 5′-GACTTCGGAGAATGAGGAGGATG-3′ 100
5′-CGATTTGTGCTTTTTCAGCAGC-3′
Pax4 5′-GCACTGGAGAAAGAGTTTCAGCG-3′ 100
5′-AAACCCTCACCGTGTCTTCAGG-3′
Pax6 5′-GAAGCGGAAGCTGCAAAGAAATAG-3′ 100
5′-GGCAAACACATCTGGATAATGGG-3′
Pdx1 5′-GCCCTGAGCTTCTGAAAACTTTG-3′ 100
5′-CCCAGGTTGTCTAAATTGGTCCC-3′
Rfx6 5′-CACCCTGCATCAAGCCTCTATG-3′ 100
5′-CACAACTGCCACCAAAGAAGTCTC-3′
Glucose 6-phosphatase 5′-CTGTGGGCATCAATCTCCTCTG-3′ 121
5′-TTGCTGTAGTAGTCGGTGTCCAGG-3′
Liver Glycogen Phosphorylase (PYGL) 5′-CGACAATGGCTTCTTTTCTCCC-3′ 5′-ACTTGACATAGGCTTCGTAGTCTGC-3′ 113