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MALDI images of porcine skin after homogenous deposition of a layer of bleomycin. (a) TIC image. Outside the tissue, MALDI matrix peaks contribute greatly to the TIC. (b) The endogenous lipid PC(34:1) (m/z 782.5672), normalized to the TIC. The different histological regions in the skin are marked. (c) Bleomycin A2, not normalized (m/z 1414.5176). (d) Bleomycin A2, normalized to TIC (m/z 1414.5176). The image was acquired with 50 µm pixel size.
