Figure 8.

Comparison of ensemble models of P_FL and of the 2P−2N N_∆23⁰−P_FL complex. (A) Full-length P dimers. Representative ensemble of the five conformers of dimeric P_FL selected by GAJOE to reproduce SAXS data [17]. Each P protomer is colored from N-terminus (blue) to C-terminus (red) and the conformers were aligned by superimposing their individual dimerization domain. The semi-transparent red sphere has a radius of 4.9 nm corresponding to the radius of gyration determined from SAXS data. (B) 2P−2N N_∆23⁰−P_FL complex. Representative ensemble of the four conformers of the 2P−2N N_∆23⁰−P_FL complex selected by GAJOE to reproduce SAXS data (Figure 7E). Each P protomer is colored from N-terminus (blue) to C-terminus (red), the N_∆23 molecules are shown in gray and the conformers were aligned by superimposing their individual dimerization domain. The semi-transparent red sphere has a radius of 5.1 nm corresponding to the radius of gyration determined from SAXS data.