FIGURE 5.

Thermal Proteome Profiling Workflow. Cells are snap frozen via liquid nitrogen for cell extraction, followed by ultra‐centrifugation. The supernatant is then subjected to treatment either with a control or drug with further exposure via a thermal cycler at varying temperatures. The heated samples are ultracentrifuged again with enzymatic digestion of the supernatant. Once peptides are formed, they are labeled with isobaric tags and combined at equal concentrations prior to cleanup steps and LC‐MS/MS analysis