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. Author manuscript; available in PMC: 2022 Dec 23.
Published in final edited form as: Mol Cancer Ther. 2018 Nov 27;18(2):278–288. doi: 10.1158/1535-7163.MCT-18-0456

Figure 1: UNC2025 inhibits MERTK activation and downstream signaling in melanoma cell lines.

Figure 1:

A) Cultures of the indicated cell lines were treated with UNC2025 for 90 minutes, and then with pervanadate phosphatase inhibitor for 3 minutes. MERTK was immunoprecipitated from cell lysates, and phosphorylated and total MERTK proteins were detected by immunoblot. B) Serum starved cultures of the indicated cell lines were treated with UNC2025 for 90 min and then stimulated with 200 nM GAS6 ligand or an equivalent volume of vehicle for an additional 10 min. Cell lysates were prepared and the indicated phosphorylated (denoted by p-) and total proteins were detected by immunoblot. ACTIN is shown as a loading control. C) The indicated cell lines were treated with UNC2025 for 72 h. Cell lysates were prepared and SURVIVIN was detected by immunoblot. Data are representative of at least 3 independent experiments.