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. 2022 Jul 21;66(18):2200164. doi: 10.1002/mnfr.202200164

Figure 1.

Figure 1

R. hominis reduced microglial activation in germ‐free rats. A) Experimental design of the R. hominis treatment. B) Representative images of Iba‐1‐stained microglia and the microglia count in the anterior cingulate cortex‐rostral (ACC‐R), ACC‐caudal (ACC‐C), dorsal hippocampus (DH), and spinal dorsal horn L6‐S1 of rats detected by immunofluorescence staining. Scale bar = 50 µm. n = 6–9. Microglia, green; nuclei, blue. One‐way ANOVA with Tukey's post‐hoc test. C) Representative three‐dimensional reconstructions of Iba‐1‐stained microglia and morphological parameters of microglia in the ACC‐R, ACC‐C, DH, and spinal cord. Scale bar = 10 µm. n = 6–9. One‐way ANOVA with Tukey's post‐hoc test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.