Figure 3.

Incubation with watermelon extracellular vesicles alters the basal secretome of Caco‐2 cells. a) Basal medium was collected from Caco‐2 cells incubated for 240 min in the presence or absence of 2.5 × 10¹⁰ watermelon extracellular vesicles (WMEV) mL⁻¹ in the apical medium compartment (n = 3). The profile of proteins in basal medium was determined by mass spectrometry; 40 proteins were detected. Data were analyzed using undirected hierarchical clustering analysis using Euclidean clustering and complete linkage. Protein profiles were then compared by isomap analysis with b) labeling of treatment group or c) unbiased labeling using k‐means clustering (two groups detected using an elbow plot).