Figure 1.

CHIKV replicates more efficiently in mouse C8-D1A astrocytic cells than NSC-34 neuronal cells. Cells were infected with CHIKV 181/25 at an MOI of 5 as measured by plaque assay on Vero cells. (A) Production of infectious virus by C8-D1A cells and NSC-34 cells. Data are presented as the geometric mean +/− SD from two independent experiments each performed in triplicate. *** p < 0.001 (B) Representative immunoblots for expression of nsP3 6, 12, 24 and 36 h post infection (hpi) of C8-D1A (top) and NSC-34 (bottom) cells. (C) Amounts of nsP3 produced in C8-D1A compared to NSC-34 cells after infection. Blots from three separate experiments were scanned, quantified with Image J, band densities normalized to actin, and ratios compared with Student’s t test. *** p < 0.001; **** p < 0.0001.