Table 3.

Primer sequences for the Semi nested consensus PCR for the amplification of fragment of the glycoprotein B according to Chmielewicz et al. (3a) and Degenerate/Consensus primers and Deoxyinosine-substituted primers used by Ehlers et al. in the gB nested PCR (3b). I^a = Deoxyinosine.

3a. Semi-Nested PCR for the Glycoprotein B (gB) Gene
First PCR
Forward	Reverse
702 Gb: 5′-CAR IaTIa CAR TWT GCM TAY GAC-3′	702 Gb: 5′-GTA RTA RTT RTA YTC YCT RAA-3′
Second PCR
Forward	Reverse
734 gB: 5′-GCA AAA TCA ACC CTA CVA GYG TNA TG-3′	702 gB: 5′-GTA RTA RTT RTA YTC YCT RAA-3′
Approximate product: 515bp
3b. Nested PCR for the Glycoprotein B (gB) Gene
First PCR
Forward	Reverse
GH1 2759: 5′-CCT CCC AGG TTC ART WYG CMT AYG A-3I	GH1 2762: 5′-CCG TTG AGG TTC TGA GTG TAR TAR TTR TAY TC-3′
GH2 3029: 5′-CCC AGT TGC ART WYG GC(N/Ia) TAY GA-3′	GH2 3033: 5′-GCC AGG CGT TGC GT(N/Ia) TAR TAR TTR TA-3′
Second PCR
Forward	Reverse
GH1 2760: 5′-AAG ATC AAC CCC AC(N/Ia) AG(N/Ia) GT(N/Ia) ATG-3′	GH1 2761: 5′-GTG TAG TAG TTG TAC TCC CTR AAC AT(N/Ia) GTY TC-3′
GH2 3031: 5′-CAA GAT TAA CCC CAC (N/Ia)AG (N/Ia)GT (N/Ia)AT G-3′	GH2 3032: 5′-TTG CGT GTA GTA GTT GTA YTC (N/Ia)CT RAA CAT-3′

Approximate product GH1: 500 bp; Approximate product GH2: 350 bp.