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SDS-PAGE analysis of rSG3PDH purification. Lane 1, uninduced bacterial extract. Induced bacterial extract (lane 2) was sonicated, and the supernatant (lane 3) was applied to a HiTrap column. Column flowthrough (lane 4) and purified rSG3PDH eluate (lane 5) are also shown. On the left are migration positions of prestained SDS-PAGE standards (103, 76, 49, 33.2, and 28 kDa; Bio-Rad, Richmond, Calif.).
