TABLE 1.

Proliferation and production of IL-4 and IFN-γ by PBMC from S. mansoni group donors stimulated in vitro with rSG3PDH or parasite antigens^a

Donor (gender, age [yr])	Proliferation (mean cpm ± SD) in response tob:				IL-4 (pg/ml) in response to:			IFN-γ (pg/ml) in response to:
	Medium	rSG3PDH	SAWAm	SEAm	rSG3PDH	SAWAm	SEAm	rSG3PDH	SAWAm	SEAm
Control
F, 30	193 ± 45	184 ± 4	913 ± 33	147 ± 74	—c	NDd	ND	—	ND	ND
M, 40	258 ± 107	236 ± 57	579 ± 123	310 ± 151	5	—	—	—	—	—
Susceptible
M, 37	137 ± 45	126 ± 61	4,413 ± 755	336 ± 16	—	—	—	—	—	—
F, 30	372 ± 197	209 ± 28	6,856 ± 564	2,580 ± 191	15	20	5	—	71.8	—
M, 58	1,500 ± 699	137 ± 16	13,404 ± 2,136	8,013 ± 1,683	—	—	—	40.8	120	81.2
M, 37	727 ± 295	249 ± 25	13,433 ± 2,887	3,606 ± 1,665	—	—	5	—	—	—
M, 24	409 ± 75	280 ± 36	55,579 ± 6,604	12,377 ± 769	—	—	—	—	25	5
Resistant
M, 30	163 ± 15	290 ± 10	21,780 ± 4,983	6,098 ± 330	—	—	—	—	—	—
M, 28	123 ± 48	292 ± 56	20,414 ± 7,305	3,500 ± 296	—	—	—	5	20	—
M, 18	199 ± 39	681 ± 46	12,500 ± 4,671	4,365 ± 1,097	—	—	—	—	30	—
M, 30	351 ± 126	2,343 ± 383	99,441 ± 11,259	34,346 ± 2,713	15	ND	ND	92.7	ND	ND
M, 34	1,641 ± 534	9,767 ± 3,093	43,716 ± 3,571	24,774 ± 1,198	5	ND	ND	71.8	ND	ND

^aRepresentative male (M) and female (F) control donors and individuals showing extremes of susceptibility or resistance after parasitological cure of previous infection with S. mansoni were assayed for proliferation and cytokine response of PBMC following in vitro stimulation with rSG3PDH, SAWA_m, and SEA_m. 

^bSignificance of differences between means of antigen-free and test cultures was evaluated by Student's t test, and cultures showing positive responses (P < 0.05 to <0.0001) are underlined. 

^c—, <5 pg/ml. 

^dND, not done.