TABLE 2.

Proliferation and production of IL-4 and IFN-γ by PBMC from S. haematobium group donors stimulated in vitro with rSG3PDH or parasite antigens^a

Donor (gender, age [yr])	Proliferative (mean cpm ± SD) in response tob:				IL-4 (pg/ml) in response to:			IFN-γ (pg/ml) in response to:
	Medium	rSG3PDH	SAWAh	SEAh	rSG3PDH	SAWAh	SEAh	rSG3PDH	SAWAh	SEAh
Control
M, 25	247 ± 34	94 ± 11	153 ± 21	101 ± 34	—c	—	—	—	—	—
M, 20	68 ± 11	47 ± 8	71 ± 26	80 ± 36	—	—	—	—	—	—
M, 40	73 ± 20	126 ± 38	112 ± 22	NDd	—	—	ND	—	—	ND
Susceptible
M, 25	105 ± 26	48 ± 19	828 ± 301	1,814 ± 176	—	—	58	—	—	12
M, 28	46 ± 18	41 ± 3	403 ± 55	734 ± 81	—	—	20	—	—	5
M, 15	126 ± 33	88 ± 21	508 ± 55	827 ± 259	—	—	—	—	—	—
M, 30	97 ± 31	57 ± 10	894 ± 290	196 ± 248	—	—	—	—	—	50
M, 16	106 ± 20	171 ± 21	2,109 ± 616	ND	—	25	ND	—	95	ND
Resistant
M, 30	56 ± 12	156 ± 11	651 ± 100	596 ± 42	—	—	—	15	28	48
M, 24	21 ± 7	121 ± 38	138 ± 15	ND	5	5	ND	8	—	ND
M, 26	122 ± 30	201 ± 8	711 ± 145	ND	—	—	ND	70	65	ND
M, 55	75 ± 28	125 ± 9	388 ± 90	ND	—	—	ND	220	10	ND
M, 40	68 ± 30	168 ± 48	577 ± 125	ND	12	10	ND	95	470	ND

^aRepresentative male (M) and female (F) control donors and individuals showing extremes of susceptibility or resistance after parasitological cure of previous infection with S. haematobium were assayed for proliferation and cytokine response of PBMC following in vitro stimulation with rSG3PDH, SAWA_h, and SEA_h. 

^bSignificance of differences between means of antigen-free and test cultures was evaluated by Student's t test, and cultures showing positive responses (P < 0.05 to <0.0001) are underlined. 

^c—, <5 pg/ml. 

^dND, not done.