TABLE 2.
Donor (gender, age [yr]) | Proliferative (mean cpm ± SD) in response tob:
|
IL-4 (pg/ml) in response to:
|
IFN-γ (pg/ml) in response to:
|
|||||||
---|---|---|---|---|---|---|---|---|---|---|
Medium | rSG3PDH | SAWAh | SEAh | rSG3PDH | SAWAh | SEAh | rSG3PDH | SAWAh | SEAh | |
Control | ||||||||||
M, 25 | 247 ± 34 | 94 ± 11 | 153 ± 21 | 101 ± 34 | —c | — | — | — | — | — |
M, 20 | 68 ± 11 | 47 ± 8 | 71 ± 26 | 80 ± 36 | — | — | — | — | — | — |
M, 40 | 73 ± 20 | 126 ± 38 | 112 ± 22 | NDd | — | — | ND | — | — | ND |
Susceptible | ||||||||||
M, 25 | 105 ± 26 | 48 ± 19 | 828 ± 301 | 1,814 ± 176 | — | — | 58 | — | — | 12 |
M, 28 | 46 ± 18 | 41 ± 3 | 403 ± 55 | 734 ± 81 | — | — | 20 | — | — | 5 |
M, 15 | 126 ± 33 | 88 ± 21 | 508 ± 55 | 827 ± 259 | — | — | — | — | — | — |
M, 30 | 97 ± 31 | 57 ± 10 | 894 ± 290 | 196 ± 248 | — | — | — | — | — | 50 |
M, 16 | 106 ± 20 | 171 ± 21 | 2,109 ± 616 | ND | — | 25 | ND | — | 95 | ND |
Resistant | ||||||||||
M, 30 | 56 ± 12 | 156 ± 11 | 651 ± 100 | 596 ± 42 | — | — | — | 15 | 28 | 48 |
M, 24 | 21 ± 7 | 121 ± 38 | 138 ± 15 | ND | 5 | 5 | ND | 8 | — | ND |
M, 26 | 122 ± 30 | 201 ± 8 | 711 ± 145 | ND | — | — | ND | 70 | 65 | ND |
M, 55 | 75 ± 28 | 125 ± 9 | 388 ± 90 | ND | — | — | ND | 220 | 10 | ND |
M, 40 | 68 ± 30 | 168 ± 48 | 577 ± 125 | ND | 12 | 10 | ND | 95 | 470 | ND |
Representative male (M) and female (F) control donors and individuals showing extremes of susceptibility or resistance after parasitological cure of previous infection with S. haematobium were assayed for proliferation and cytokine response of PBMC following in vitro stimulation with rSG3PDH, SAWAh, and SEAh.
Significance of differences between means of antigen-free and test cultures was evaluated by Student's t test, and cultures showing positive responses (P < 0.05 to <0.0001) are underlined.
—, <5 pg/ml.
ND, not done.