Figure 2.

Seven‐point dose‐response curve of EGCG A), gallocatechin B), gallic acid C), and epicatechin gallate D) on HCT116 EGFP spheroids in aggregation and epicatechin gallate on formed spheroids E). Spheroids were exposed to increasing concentrations from 3 to 100 µM for 72 h. Spheroid integrity was quantified by the High Content Imaging System Operetta (Perkin Elmer) and expressed as a percentage of SI of vehicle‐treated controls (DMSO 0.05%). IC₅₀ was calculated applying dose–response‐inhibition nonlinear regression analysis. Data are expressed as mean ± SEM, and all experiments were performed from at least four independent experiments. Spheroids were imaged on High Content Imaging System Operetta (Perkin Elmer) with 10XLWD objective in brightfield and green fluorescent channels (merged images) at different concentrations. Scale bars, 200 µm.