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. 2022 Dec 18;23(24):16163. doi: 10.3390/ijms232416163

Figure 3.

Figure 3

Optical density (OD) measurement at 600 nm and crystal violet (CV) biofilm quantification. CFSs were assayed to determine their ability in preventing pathogen biofilm formation through OD600 measurements for (a) A. actinomycetemcomitans, (b) S. mitis, and (c) S. mutans; and CV quantification for (d) A. actinomycetemcomitans, (e) S. mitis, and (f) S. mutans. Data are represented as the mean of three independent experiments ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. OD 600 nm = optical density at 600 nm; Abs 570 nm = absorbance at 570 nm; CFSs = cell-free supernatants.