Figure 4.

Temperature dependence of ALOX activity of the randomly selected bony fish ALOX isoforms. Activity assays were carried out using the cellular lysate supernatants as enzymes source. Aliquots of the lysate supernatants were incubated in the standard activity assays with AA as substrate at different temperatures and the amounts of specific ALOX products formed during a 3 min incubation period were quantified by RP-HPLC (see Section 4). At each temperature two independent incubations were carried out. Means and error bars are shown, and curves were calculated by non-linear regression (A) N. furzeri, (B) P. nyererei, (C) S. formosus.