Skip to main content
. 2001 Jan;69(1):252–261. doi: 10.1128/IAI.69.1.252-261.2001

FIG. 1.

FIG. 1

(A) SDS-PAGE of purified SBR-CTA2/B (lane 1) and SBR–LT-IIaA2/B (lane 2) dissociated into the SBR-A2 subunit (∼60 kDa) and B subunit (∼12.5 kDa) monomers. (B) Western blot of recombinant SBR-CTA2/B (lane 1) and SBR-LT-IIaA2/B (lane 2) probed with polyclonal anti-SBR Abs and of SBR-CTA2/B (lane 3) and SBR-LT-IIaA2/B (lane 4), using polyclonal Abs to CT or LT-IIa, respectively. The positions of the molecular mass markers (kilodaltons) are indicated. (C) Titration of purified SBR–LT-IIaA2/B in a GD1b-ELISA using polyclonal Abs to SBR and LT-IIa antigenic determinants. Each point represents the mean OD of triplicate values.