TABLE 1.
Proliferation and CD4+ T cell apoptosis following cell depletions
| Cells depleted | 8-wk infection
|
16-wk infection
|
||
|---|---|---|---|---|
| Proliferationa (cpm, 10−4) | Apoptosisb (% annexin V+) | Proliferation (cpm, 10−4) | Apoptosis (% annexin V+) | |
| None (mock depletion) | 2.9 ± 0.1 | 29.2 ± 2.2 | 1.9 ± 0.1 | 33.6 ± 6.4 |
| CD19 | 2.9 ± 0.2 | 15.1 ± 1.1 | 2.1 ± 0.9 | 19.9 ± 0.8 |
| CD8 | 2.8 ± 0.2 | 31.8 ± 2.8 | 1.7 ± 0.1 | 35.2 ± 4.9 |
| CD8, CD19 | 3.5 ± 0.1 | 13.1 ± 1.4 | 2.3 ± 0.3 | 21.2 ± 3.5 |
a
Uptake of [3H]thymidine by splenocytes at 120 h of culture in the presence of SEA (10 μg/ml). The data are counts per minute ± the standard deviation of six replicate samples.
b
Annexin V binding of CD4+ PI− cells measured at 60 h of culture in the presence of SEA (10 μg/ml). The data are mean percent annexin V+ cells among viable CD4+ T cells ± the standard deviation of six replicates.