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. 2022 Dec 3;14(12):2711. doi: 10.3390/v14122711

Figure 1.

Figure 1

Nodavirus genome organization and replication. (A) The bipartite FHV RNA genome encodes the viral replicase protein A, the key protein for viral RNA replication, on RNA1 and the capsid protein precursor on RNA2. Subgenomic RNA3, transcribed from RNA1, encodes protein B1, identical to the C-terminus of protein A, and protein B2 which is an RNAi suppressor. (B) Cryo-EM tomogram of Drosophila S2 cells with nodavirus replication complexes: invaginations (spherules) of the outer mitochondrial membrane into a vastly dilated intermembrane space. Arrowheads: black = outer mitochondrial membrane; white = inner mitochondrial membrane; red = spherules, filled with dsRNA; yellow = crown-like structures at the spherule open necked connections to the cytoplasm. (C) Tomographic reconstruction of the 12-fold symmetrical nodavirus replication complex crown, from reference [24].