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. 2022 Dec 19;23(24):16176. doi: 10.3390/ijms232416176

Figure 1.

Figure 1

Effect of 6-OHDA, EPA, and DHA (alone or in combination) on the viability of SH-SY5Y cell line. Cells were treated for 24 h using BSA/NaCl solution as vehicle treatment condition (A) and different concentrations of 6-OHDA (B), EPA (C), or DHA (D). Based on this result, 50 μM 6-OHDA was used to treat the cells together with EPA (E) or DHA (F). In the last two cases, the first column represents untreated cells (white), the second column is the viability after 50 μM 6-OHDA treatment (black) and all other columns show co-treatment with 50 μM 6-OHDA and two omega-3 fatty acids. Cell viability was assessed using MTT assay. Data from three independent experiments using four independent cultures per condition were analyzed by ANOVA using Graphpad Prism software. * p < 0.01, ** p < 0.001, and *** p < 0.0001.