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Effects of 6-OHDA, EPA, DHA, and 5-AZA on the apoptosis of SH-SY5Y cell line. Cells were treated for 24 h using 50 μM 6-OHDA, 100 μM EPA, 100 μM DHA, and 1 μM 5-AZA. Cells were harvested, stained with PI, and analyzed using flow cytometry. Representative plots are reported together with means ± S.D. Data from three independent experiments using four independent cultures per condition were analyzed by ANOVA using Graphpad Prism software *** p < 0.0001.
